Establishment of an imaging-based screening pipeline for the identification of human ribosome biogenesis inhibitors
The established screening pipelines provide a robust, efficient, and sensitive experimental framework to identify chemical compounds that impair ribosome synthesis. The combination of readouts allows to distinguish effects on pre-rRNA synthesis from downstream effects on ribosome assembly. Established counter assays on DNA damage and protein degradation enable to exclude effects on these pathways, which commonly interfere with ribosome synthesis indirectly. The developed assays are easily scalable to screen libraries of higher complexity in the future.
Authors
- Réka Hollandi (ORCID: https://orcid.org/0000-0002-4052-6846)
- Ivo Zemp (ORCID: https://orcid.org/0000-0003-1549-9871)
- Péter Horváth (ORCID: https://orcid.org/0000-0002-4492-1798)
- Ulrike Kutay (ORCID: https://orcid.org/0000-0002-8257-7465)
- Kerstin Dörner (ORCID: https://orcid.org/0000-0003-2541-0930)
- Claudia Gafko
- Matteo Rosellini (ORCID: https://orcid.org/0000-0002-3141-6768)
Institutions
- University of Basel (CH)
- HUN-REN Szegedi Biológiai Kutatóközpont (HU)
- ETH Zurich (CH)
- Institute for Biomedical Engineering (CH)
- Life Science Zurich (CH)
Publication Details
- Journal
- BMC Biology
- Published
- 2025-10-21
- DOI
- https://doi.org/10.1186/s12915-025-02425-2
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Branco Weiss Fellowship – Society in Science
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Horizon 2020 Framework Programme