The metabolites and metabolic pathways of formaldehyde in the roots, stems and leaves of <i>Epipremnun aureum</i>

The removal of formaldehyde by plants has the characteristics of low cost, low energy consumption and green environmental protection. Existing studies on eliminating formaldehyde by plants mainly focused on the macroscopic removal performance of plants; however, the metabolites and metabolic pathways of detoxicating formaldehyde by plants such as Epipremnun aureum (E. aureum) had not been revealed. In this study, the isolated roots, stems and leaves of E. aureum were treated with the time gradient and concentration gradient of H 13 CHO solution, and the metabolites of detoxicating formaldehyde in each part of E. aureum were determined by nuclear magnetic resonance technology. The metabolic pathway of formaldehyde in each part of E. aureum was analysed. The results showed that the metabolites of assimilating formaldehyde by various parts of E. aureum included formic acid (FA), asparagine, glutamic acid, glycine, glutamine, citric acid, fructosamine and gluconic acid. The glyoxylic acid cycle and TCA cycle were synergistic in the process of formaldehyde metabolism in various parts of E. aureum . The Calvin cycle existed in the process of formaldehyde metabolism in the stem and leaves, and the C1 cycle played a role in the process of formaldehyde metabolism in the root of E. aureum .