Serodiagnosis of amoebic abscess: a retrospective diagnostic accuracy study of kits marketed in Europe
Amoebiasis caused by <i>Entamoeba histolytica</i> is common in countries with low socio-economic levels. The most lethal form is extraintestinal amoebiasis, mainly liver abscesses, which require rapid and accurate diagnosis. Diagnosis is based on clinical, radiological, and, most importantly, serological tests. Implementing a diagnostic strategy requires both knowledge of how diagnostic tests compare with each other and thorough cross-validation. To help the clinical microbiologist choose a serological reagent, we evaluated the performance of four serological reagents currently available on the European market using a large biobank of sera from seven French university hospitals. In addition to patients with amoebic abscess and healthy donors, our study included many samples from patients with other parasitic and non-parasitic pathologies (liver diseases, immune dysfunctions) in order to study particularly non-specific reactivities.
Authors
- Frédéric Gabriel (ORCID: https://orcid.org/0000-0002-9223-2516)
- Frédéric Grenouillet (ORCID: https://orcid.org/0000-0001-6001-3135)
- Pauline Tirard-Collet (ORCID: https://orcid.org/0000-0001-6365-8174)
- F. Touafek
- Clotilde Amiot (ORCID: https://orcid.org/0000-0001-8496-2968)
- Alicia Moreno‐Sabater (ORCID: https://orcid.org/0000-0002-7571-9533)
- Marie‐Pierre Brenier‐Pinchart (ORCID: https://orcid.org/0000-0003-1366-5856)
- Hélène Fricker‐Hidalgo (ORCID: https://orcid.org/0000-0002-1932-2531)
- Anthony Marteau (ORCID: https://orcid.org/0009-0006-0495-1091)
- Damien Dupont (ORCID: https://orcid.org/0000-0002-6992-1073)
- Harry Sokol (ORCID: https://orcid.org/0000-0002-2914-1822)
- Emma Prétot
- L. Delcey
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Inserm (FR)
- AgroParisTech (FR)
- Centre Hospitalier Universitaire de Grenoble (FR)
- Microbiologie de l’alimentation au service de la santé (FR)
- Centre Hospitalier Universitaire de Bordeaux (FR)
- Sorbonne Université (FR)
- Centre Hospitalier Universitaire de Besançon (FR)
- Hôpital de la Croix-Rousse (FR)
- Université Sorbonne Paris Nord (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Hospices Civils de Lyon (FR)
- Laboratoire Chrono-Environnement (FR)
- Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine (FR)
- Centre d'Immunologie et des Maladies Infectieuses (FR)
- Centre de Recherche Saint-Antoine (FR)
- Hôpital Charles-Foix (FR)
- Université Marie et Louis Pasteur (FR)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- Journal of Clinical Microbiology
- Published
- 2025-10-21
- DOI
- https://doi.org/10.1128/jcm.00179-25
- Primary Topic
- Amoebic Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00