Analysis and identification of coumarins in different parts of Chimonanthus salicifolius and biosynthetic pathways prediction

A total of 200 compounds were identified, of which 69.04% were automatically annotated using self-built R script, effectively accelerating the identification of target compounds, and significantly improving the efficiency of compound structure annotation. The biosynthetic pathway of coumarins was predicted on basis of the identified compounds. Subsequently, a remarkable distinction of metabolites was observed from the shells of seed, leaves and seeds compared to the roots and branches, followed by a relatively minor disparity from branches and roots through PCA analysis and PLS-DA analysis. It was revealed in the heatmap that coumarins, flavonoids, terpenoids, atty acids and cinnnamic aicds were abundant in the leaves of <i>C. salicifolius</i>, providing material basis for subsequent pharmacological research.