The acute toxicity, antioxidant and antihyperlipidemic activity of flavonoid glycosides from Ruta montana in female rats with hyperlipidemia induced by Triton WR-1339

This work aimed to evaluate the antihyperlipidemic activity of the aerial part of Ruta montana flavonoid- glycosides enriched extract (RMFEE) in Triton WR1339-induced hyperlipidemia in female rats, along with its antioxidant properties and acute toxicity. Female Wistar rats with Triton WR-1339-induced hyperlipidemia were used to assess RMFEE's antihyperlipidemic effects. The DPPH assay was used to determine antioxidant activity and acute toxicity was assessed via a single oral gavage of RMFEE at 600 and 2000 mg/kg body weight, following OECD 423 guidelines. Pretreatment with RMFEE at 150 and 400 mg/kg significantly reduced elevations in plasma levels of total cholesterol, triglycerides and non-HDL-C, while also significantly decreasing the atherogenic TG/HDL-C ratio in the hyperlipidemic rats. RMFEE also exhibited notable antioxidant activity in the DPPH assay. No mortality or signs of toxicity were observed within two weeks following a single oral administration of RMFEE at either 600 or 2000 mg/kg. Oral administration of RMFEE (150 and 400 mg/kg) prior to induction of hyperlipidemia by Triton WR-1339 demonstrated antihyperlipidemic activity in female rats. Specifically, the 400 mg/kg dose significantly prevented the increase in the TG/HDL ratio caused by Triton WR-1339. RMFEE also showed antioxidant activity with IC50=88.27 ± 3.12 µg/mL. The acute toxicity of the oral dose for RMFEE indicates very low toxicity, with an LD50 exceeding 2000 mg/kg.