Alpha-centractin is a novel substrate of SETD3 methyltransferase <i>in vitro</i>
Fluorography revealed that SETD3 methylates at least five novel proteins besides β-actin in HAP1 cells. TurboID proximity labeling identified α-centractin, a key dynactin subunit, as an SETD3 interactor and an <i>in vitro</i> methylation target, suggesting that SETD3 potentially regulates not only actin cytoskeleton dynamics but also dynein-mediated intracellular transport.
Authors
- Michał Zaród (ORCID: https://orcid.org/0000-0001-6580-1608)
- K. Ślusarczyk
- Takao Ishikawa (ORCID: https://orcid.org/0000-0002-3558-0880)
- Jakub Drożak (ORCID: https://orcid.org/0000-0002-3601-3845)
- Apolonia Witecka (ORCID: https://orcid.org/0000-0002-7171-3222)
- Joanna Kamińska (ORCID: https://orcid.org/0000-0002-0168-7882)
- Paulina Emmel
Institutions
- Postgraduate School of Molecular Medicine (PL)
- University of Warsaw (PL)
Publication Details
- Journal
- PeerJ
- Published
- 2025-10-20
- DOI
- https://doi.org/10.7717/peerj.20195
- Primary Topic
- Ubiquitin and proteasome pathways
- Type
- article
- Field-Weighted Citation Impact
- 0.00