CRISPR/Cas Tools for the Detection of Borrelia sensu lato in Human Samples
The Cas12/crRNA-based system offers a sensitive and accessible alternative to qPCR, especially in settings lacking real-time PCR instrumentation. Future developments may include integration with isothermal amplification and microfluidic platforms to enhance direct detection capabilities.
Authors
- Ermanno Nardon
- Serena Bonin (ORCID: https://orcid.org/0000-0002-7349-0296)
- Dino Paladin (ORCID: https://orcid.org/0009-0004-2996-1195)
- Eros Azzalini (ORCID: https://orcid.org/0000-0002-5193-268X)
- D. Boscarino (ORCID: https://orcid.org/0009-0003-5774-1723)
Institutions
- University of Trieste (IT)
- Ab Analitica (Italy) (IT)
Publication Details
- Journal
- Genes
- Published
- 2025-10-18
- DOI
- https://doi.org/10.3390/genes16101233
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Regional Development Fund