TF-343 Recovered Cyclophosphamide-Induced Compromised Immune Status in a Mouse Model

Cyclophosphamide (CP) exerts potent cytostatic and immunosuppressive effects by inhibiting the proliferation and function of tumor and immune cells, thereby being widely used for therapeutic approaches and molecular mechanism studies in immunocompromised models and autoimmune disease models. TF-343 is a mixed extract derived from medicinal plants with anti-inflammatory and antioxidant properties and exhibits anti-inflammatory activity by modulating signaling pathways associated with immune responses. To elucidate the potential immunomodulatory effects and underlying mechanisms of TF-343 in more detail, we investigated its efficacy in a CP-induced immunosuppressive mouse model. Administered TF-343 significantly recovered CP-induced decreases in body weight and organ indices, including those of the thymus and spleen. The CP-mediated decrease in the number of lymphocytes and white blood cells and immunoglobulin production were restored by TF-343 administration. Furthermore, TF-343 enhanced the proliferation of total splenocytes and their subpopulations, including T and B lymphocytes. The administration of TF-343 promoted the function of natural killer cells and macrophages in CP-induced immunocompromised mice. Additionally, TF-343 supplementation reverted the CP-elicited downregulation of interleukin 2 and interferon γ mRNA and protein expression in splenocytes. Therefore, our results demonstrate that TF-343 enhances immune function and has potential as an immunoregulatory substance.