Hepatocyte dedifferentiation in 2D culture reveals extensive transcriptomic and proteomic rewiring
Collectively, primary mouse hepatocytes in culture rewire the transcriptome and proteome, which may affect the utility of this model to study physiological and molecular mechanisms related to the liver. We developed the Shiny app "Hepamorphosis" (https://cbmr.ku.dk/research/resources/shiny-apps/), which allows users to explore RNA/protein correlations, zonation profiles, and cell-type-specific transcription in full liver and cultured hepatocytes.
Authors
- Ben Stocks (ORCID: https://orcid.org/0000-0002-9281-0793)
- Jonas T. Treebak (ORCID: https://orcid.org/0000-0003-1488-7012)
- Daniel T. Cervone (ORCID: https://orcid.org/0000-0001-5675-4985)
- Morten Dall (ORCID: https://orcid.org/0000-0002-8577-3162)
- Atul S. Deshmukh (ORCID: https://orcid.org/0000-0002-2278-1843)
Institutions
- University of Copenhagen (DK)
- Qualcomm (United Kingdom) (GB)
- Novo Nordisk Foundation (DK)
Publication Details
- Journal
- Hepatology Communications
- Published
- 2025-10-07
- DOI
- https://doi.org/10.1097/hc9.0000000000000795
- Primary Topic
- Liver physiology and pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.00