Association of PDGFRA gene Polymorphisms SNP with Myopia in Najaf Governorate

Background: Myopia is a growing global visual disorder and a significant public health burden. The development of this condition is influenced by genetic and environmental factors. The aim of this study to investigate the potential association between the +217(G>A) SNP in the platelet-derived growth factor receptor alpha (PDGFRA) gene and myopia in the population of Najaf Governorate. Methodology This study was conducted on 106 blood samples, including 66 patients with myopia and 40 healthy volunteers as a control group. Genomic DNA was extracted from all samples, and the target region of the PDGFRA gene was amplified using polymerase chain reaction (PCR). Genotypes were then determined using SspI-based restriction fragment length polymorphism (RFLP-PCR). The results were then analyzed statistically. Results: The results revealed a statistically significant difference in the distribution of genotypes and alleles of this gene between patients and healthy controls, with the mutant allele being significantly more frequent in patients with myopia than in the control group (P<0.05, meaning there is a relationship between the SNP +217(G>A) polymorphism and an increased risk of myopia in this population under study. Conclusion :The study concluded that the SNP +217(G>A) polymorphism in the PDGFRA gene may be a potential biomarker of genetic predisposition to myopia. Although these results are preliminary due to the small sample size used, the study recommends conducting further studies and expanding the number of samples to confirm these results and provide a greater and deeper understanding of the genetic mechanisms behind this relationship.