Optimization and comparison of genomic DNA extraction from whole blood collected in PAXgene blood RNA tube using automated platforms
Not all magnetic bead-based automated platforms are suitable for gDNA extraction from blood samples collected in PAXgene Blood RNA Tubes. Systematic pre-treatments optimization provides guidance for routine and high-throughput workflows, and storage conditions and freeze-thaw cycles offer practical reference for biobanking.
Authors
- Tomoe Shiomi (ORCID: https://orcid.org/0009-0005-7611-8528)
- Jianlan You (ORCID: https://orcid.org/0000-0002-2129-1500)
- André L. Moreira (ORCID: https://orcid.org/0000-0003-1857-3774)
- Sandra Mendoza
- Luis Chiriboga (ORCID: https://orcid.org/0000-0002-2028-6873)
- Paul Zappile
Institutions
- New York Genome Center (US)
- New York University (US)
Publication Details
- Journal
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Published
- 2025-10-19
- DOI
- https://doi.org/10.1515/cclm-2025-1079
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00