Comparative Evaluation of Different Concentrations of Bovine Colostrum-based Storage Medium and HBSS in Maintaining Periodontal Cell Ligament Cell Viability: An In Vitro Study

of the tooth and reimplantation may be significantly enhanced by the selection of the transport medium. 8The modern modalities in interim storage have experimented with several storage media such as saliva, ViaSpan, Hank's balanced salt solution (HBSS), and milk that have been backed up by evidence.Several other materials and solutions are being analyzed for their potential to maintain the viability of PDL, which is crucial in the prognosis of the replanted avulsed tooth.Factors in storage mediums that can increase the IntroductIonA dental avulsion occurs when a tooth completely shifts out of its socket, severing the periodontal ligament (PDL) and potentially breaking the alveolar bone.It accounts for 0.5-3% of all traumatic injuries of the permanent dentition and is considered to be the most severe form of dental trauma that is common in society. 1 Dental trauma occurs most frequently in schools, homes, and streets, and it is more common in young adults and children. 2 The most frequent reasons why children sustain dental trauma are traumatic episodes that occur during recreational and athletic activities. 3ompared to mandibular teeth, maxillary teeth experience trauma more frequently, most likely as a result of their exposed position. 4he anterior teeth in the maxilla are considered to be of a high area of impact with higher chances of avulsion. 5The development of permanent teeth can be impacted by trauma that occurs in the oral cavity of the primary teeth, which could include staining, caries, misaligned teeth, and hypoplasia. 6he following injury types are classified as a part of dental trauma: Broken teeth, tooth fractures, such as those involving the roots, the enamel, subluxation, intrusion, avulsion, the jaw, the socket walls, and the gums, among other conditions. 7Time is an important marker that influences several factors that come together to determine whether an avulsed tooth can be successfully reimplanted.The increase in the time interval between avulsion 1,