Effects of hypothermic oxygenated machine perfusion on bile composition after liver transplantation – Findings from a randomized controlled trial
Worldwide liver allograft scarcity has led to the implementation of hypothermic oxygenated machine perfusion (HOPE) to enhance the safety of liver transplantation (LT) using extended criteria donors. However, its protective mechanisms remain incompletely understood, largely because of limited human data. In this study, we provide evidence that HOPE improves biliary lipid secretion after LT, limits intrahepatic bile acid accumulation upon allograft reperfusion, reduces the biliary bile acid to phosphatidylcholine ratio and promotes normalization of serum bile acids levels post-LT. Our findings suggest that protection from bile acid toxicity may constitute a novel mechanism underlying the effects of HOPE.
Authors
- Isabella Lurje (ORCID: https://orcid.org/0000-0002-4006-7707)
- Pavel Strnad (ORCID: https://orcid.org/0000-0003-3164-9614)
- Peri Husen
- David Meierhofer (ORCID: https://orcid.org/0000-0002-0170-868X)
- Janina Eden (ORCID: https://orcid.org/0000-0002-8724-9313)
- Marlene Kohlhepp (ORCID: https://orcid.org/0000-0002-2828-1663)
- Deniz Uluk (ORCID: https://orcid.org/0000-0001-6395-0845)
- Zoltán Czigány (ORCID: https://orcid.org/0000-0003-0373-3210)
- Philipp Dutkowski (ORCID: https://orcid.org/0000-0002-3016-604X)
- Frank Tacke (ORCID: https://orcid.org/0000-0001-6206-0226)
- Ulf P. Neumann (ORCID: https://orcid.org/0000-0002-3831-8917)
- Christoph Michalski (ORCID: https://orcid.org/0000-0003-3520-1040)
- Georg Lurje (ORCID: https://orcid.org/0000-0001-9674-0756)
- Frederik Schliephake
- Justus Pein (ORCID: https://orcid.org/0009-0003-4902-330X)
- Cornelius Engelmann
Institutions
- University Medical Center Groningen (NL)
- Heidelberg University (DE)
- University Hospital Heidelberg (DE)
- University Hospital of Basel (CH)
- Max Planck Institute for Molecular Genetics (DE)
- Charité - Universitätsmedizin Berlin (DE)
- RWTH Aachen University (DE)
Publication Details
- Journal
- JHEP Reports
- Published
- 2025-10-18
- DOI
- https://doi.org/10.1016/j.jhepr.2025.101647
- Primary Topic
- Organ Transplantation Techniques and Outcomes
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Pfizer
- AstraZeneca
- Sanofi
- Gilead Sciences
- Alnylam Pharmaceuticals
- CSL Behring
- Arrowhead Pharmaceuticals
- Deutsche Forschungsgemeinschaft
- Novo Nordisk
- Astellas Pharma
- Ipsen
- Grifols