A multifunctional bilayer wound dressing co-loaded with nanosilver and bFGF for enhanced skin regeneration: synergistic antibacterial, hemostatic, and angiogenic effects

The bilayer dressing allowed the sustained release of nanosilver and bFGF for more than 2 weeks, had good water absorption and water retention rates, had an appropriate water vapor transmission rate, absorbed excess exudate, and maintained a moist wound environment. <i>In vitro</i>, the dressing prevented bacterial penetration, accelerated fibroblast migration, and promoted angiogenesis and coagulation. Furthermore, the bilayer dressing facilitated cell migration and proliferation, promoted vessel formation, shortened the healing time and significantly promoted wound healing <i>in vivo</i>. By day 3, the wound healing rate in the bilayer dressing group (35.28% ± 2.06%) was significantly higher than that in the control group (23.99% ± 4.32%), representing an increase of approximately 47%. Our findings suggest that this multifunctional bilayer dressing coloaded with nanosilver and bFGF is a potential candidate for skin repair and regeneration applications.