Optimization of In Vitro Transcription by Design of Experiment to Achieve High Self-Amplifying RNA Integrity
This study successfully established a multivariate IVT design space fulfilling preset integrity and yield criteria, providing critical data references for the industrialization and quality specification development of saRNA vaccines.
Authors
- Xiao Ma
- Chaoying Hu
- Guanxing Liu
- Mingchen Liu (ORCID: https://orcid.org/0000-0002-0649-8976)
- Jianyang Liu (ORCID: https://orcid.org/0000-0002-8683-1109)
- Miao Xu (ORCID: https://orcid.org/0000-0002-6472-293X)
- Fan Gao (ORCID: https://orcid.org/0000-0002-7949-0828)
- Qian He (ORCID: https://orcid.org/0000-0003-4346-9899)
- Wu Xing (ORCID: https://orcid.org/0000-0002-5371-6198)
- Zhenglun Liang (ORCID: https://orcid.org/0000-0002-6740-0864)
- Zhihao Fu (ORCID: https://orcid.org/0009-0009-6116-0589)
- Qunying Mao
- Kelei Li
- Lifang Song (ORCID: https://orcid.org/0000-0002-2323-0226)
- Xuanxuan Zhang
- Qian Wang (ORCID: https://orcid.org/0009-0006-7421-1451)
- Haocheng Wang (ORCID: https://orcid.org/0009-0002-9736-1365)
Institutions
- National Medical Products Administration (CN)
- Beijing VDJBio (China) (CN)
- Changchun Institute of Biological Products (CN)
- National Institutes for Food and Drug Control (CN)
Publication Details
- Journal
- Vaccines
- Published
- 2025-10-17
- DOI
- https://doi.org/10.3390/vaccines13101062
- Primary Topic
- RNA and protein synthesis mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Chinese Academy of Medical Sciences