Novel multiplex family-wide PCR and Nanopore sequencing of amplicons (FP-NSA) approach for surveillance of influenza- and coronaviruses in humans and animals
The assay's short PCR amplicons enable screening of samples within 4 h, from PCR to sequencing and bioinformatics analysis, providing an adequate number of pathogens' reads. The portable MinION device makes the assay suitable for pathogen surveillance in disease hotspots and resource-limited regions such as low- and middle-income countries. Thus, the FP-NSA assay is a valuable tool for detecting potential novel and known zoonotic respiratory viruses in the targeted families across various host species.
Authors
- Norbert Nowotny (ORCID: https://orcid.org/0000-0002-3548-571X)
- Letizia Ceglie (ORCID: https://orcid.org/0000-0002-6056-7821)
- Sandra Revilla‐Fernández
- Christoph Leth (ORCID: https://orcid.org/0000-0002-1659-5286)
- Madhur Dhingra (ORCID: https://orcid.org/0000-0002-0624-866X)
- F. Schmoll (ORCID: https://orcid.org/0000-0002-1887-9880)
- Charles Euloge Lamien (ORCID: https://orcid.org/0000-0003-1899-7614)
- Kouramoudou Bereté
- Giovanni Cattoli (ORCID: https://orcid.org/0000-0002-9011-7135)
- William G. Dundon (ORCID: https://orcid.org/0000-0002-2776-169X)
- Adi Steinrigl (ORCID: https://orcid.org/0000-0001-7727-7508)
- Irene K. Meki (ORCID: https://orcid.org/0000-0001-5983-2088)
- Tirumala Bharani K. Settypalli (ORCID: https://orcid.org/0000-0002-9090-8471)
- Ki Bum Ahn (ORCID: https://orcid.org/0000-0003-4445-5720)
- Artem Metlin (ORCID: https://orcid.org/0000-0002-4283-0171)
Institutions
- International Atomic Energy Agency (AT)
- Food and Agriculture Organization of the United Nations (IT)
- University of Veterinary Medicine Vienna (AT)
- Korea Atomic Energy Research Institute (KR)
- Mohammed Bin Rashid University of Medicine and Health Sciences (AE)
- Istituto Zooprofilattico Sperimentale delle Venezie (IT)
- Institut de Recherche Agronomique de Guinée (GN)
- Austrian Agency for Health and Food Safety (AT)
Publication Details
- Journal
- Genome Medicine
- Published
- 2025-10-17
- DOI
- https://doi.org/10.1186/s13073-025-01550-5
- Citations
- 1
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.40
Funders
- International Atomic Energy Agency