Hollow fiber flow field-flow fractionation enables high-quality Extracellular Vesicle isolation from minimal plasma samples in polycythemia vera liquid biopsy
HF5 integrates EV isolation and characterization, enhancing efficiency, and preserving sample integrity and functionality. Its minimal sample requirement and reproducibility make it particularly suitable for clinical and translational research. Our study demonstrated HF5 as a powerful better alternative to conventional EV isolation methods, with strong potential for standardized applications in biomarker discovery and cancer research.
Authors
- Francesca Palandri (ORCID: https://orcid.org/0000-0001-8367-5668)
- Anna Placci (ORCID: https://orcid.org/0009-0005-3332-6909)
- Barbara Roda (ORCID: https://orcid.org/0000-0003-1060-2921)
- Valentina Marassi (ORCID: https://orcid.org/0000-0001-8742-3708)
- Andrea Zattoni (ORCID: https://orcid.org/0000-0001-6971-5360)
- Filippo Maltoni
- Massimiliano Bonafè (ORCID: https://orcid.org/0000-0002-5218-6551)
- Ghazal Narimanfar
- Gianluca Storci (ORCID: https://orcid.org/0000-0001-5145-3091)
- Pierluigi Reschiglian
- Lucia Catani (ORCID: https://orcid.org/0000-0002-4650-201X)
- Stefano Giordani (ORCID: https://orcid.org/0009-0005-6357-3679)
Institutions
- Istituto di Ematologia di Bologna (IT)
- Azienda USL di Bologna (IT)
- Istituto Nazionale Biostrutture e Biosistemi (IT)
- University of Bologna (IT)
Publication Details
- Journal
- Talanta
- Published
- 2025-10-17
- DOI
- https://doi.org/10.1016/j.talanta.2025.129004
- Citations
- 1
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.81