The EDEN ISS mobile test facility microbiome changes by cleaning and continued use

The results revealed that the bioburden of the different sampling positions was not significantly reduced by cleaning, indicating that the employed cleaning regime was unsuited in its current form to adequately lower the bioburden. Identification of the isolates, as well as the full microbiome, revealed mostly environmental genera. However, in both cases, genera containing plant as well as human pathogens, like <i>Pseudomonas</i> and <i>Acinetobacter</i>, were identified and accounted for up to 16.1% of all reads for a sampling condition in the case of <i>Pseudomonas</i>. The two sets of sequencing data had little overlap, with <i>Rhodococcus</i> and <i>Microbacterium</i> being the only genera shared between all sampling conditions and sequencing approaches, and emphasized different aspects of the MTF microbiome, highlighting the advantages of using a combined approach to obtain a more complete picture of the microbiome composition.