Phytotoxic Effects and Microbial Responses to Ciprofloxacin and Its Removal by Hydrilla verticillata

Ciprofloxacin (CIP), a widely used fluoroquinolone antibiotic, is frequently detected in aquatic environments, raising concerns over its ecological risks. In this study, the submerged macrophyte <i>Hydrilla verticillata</i> was employed to investigate its capacity for CIP removal and the associated ecotoxicological effects. A series of batch experiments were conducted to evaluate plant growth, photosynthetic efficiency, oxidative stress responses, CIP biodegradation pathways, and shifts in epiphytic microbial communities. Results showed that CIP significantly inhibited the growth of <i>H. verticillata</i>, with inhibition rates of plant length and fresh weight reaching 15.8% and 29.7%, respectively, at 5 mg/L. Photosynthetic parameters were severely suppressed. Fv/Fm represented the maximum quantum efficiency of PSII, significantly decreased by 94.3% at 10 mg/L, while chlorophyll a and b contents declined by up to 36.1% and 31.2%, respectively, compared to control. Antioxidant responses showed <i>H. verticillata</i> undergo peroxidation damage. Biodegradation analysis revealed that <i>H. verticillata</i> effectively removed CIP from water, with maximum removal rates of 37% at 1 mg/L and 31% at 5 mg/L under high biomass (4.2 g) conditions. CIP accumulation was higher in stems than in leaves, and three biodegradation intermediates (C306, C263, and C248) were identified, suggesting a degradation pathway involving piperazine ring cleavage, de-ethylation, and deamination. High-throughput sequencing further indicated that CIP exposure reduced bacterial diversity and richness on <i>H. verticillata</i> surfaces, while promoting antibiotic-resistant taxa such as <i>Actinobacteria</i> and <i>Bacteroidota</i>. These findings highlight the potential role of <i>H. verticillata</i> in antibiotic-contaminated water remediation.