Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse
Opn3-phiC31o KI mice were successfully generated. We can generate Opn3 null mice that does not disrupt Chml by preparing homozygotes of Opn3-phiC31o. We have deposited the sequences including the newly found transcription start site of Chml.
Authors
- Yuichi Hiraoka (ORCID: https://orcid.org/0000-0002-4492-4403)
- Yuka Nakano (ORCID: https://orcid.org/0000-0001-9983-6710)
- Satoru Moritoh
- Kenji F. Tanaka (ORCID: https://orcid.org/0000-0003-2511-0057)
- Chisato Iba
- Ziyan Ma (ORCID: https://orcid.org/0000-0002-0630-6831)
- Toshihide Kurihara (ORCID: https://orcid.org/0000-0002-5457-2720)
- R. Kwok
- Hikaru Ikuta
- Yahan Chuai
- Taichi Sayanagi
Institutions
- Keio University (JP)
- Keio University Hospital (JP)
- Institute for Advanced Medical Research (US)
- The University of Tokyo (JP)
Publication Details
- Journal
- Eye and Vision
- Published
- 2025-10-17
- DOI
- https://doi.org/10.1186/s40662-025-00455-z
- Primary Topic
- Photoreceptor and optogenetics research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science
- Core Research for Evolutional Science and Technology