Immuno-Golgi method - Integrating immunohistochemistry and Golgi staining to characterize newborn neurons morphology

Visualizing neuronal dendritic architecture while simultaneously identifying specific neuronal phenotype is challenging. Here, we present a detailed description of the Immuno-Golgi technique which combines bromodeoxyuridine (BrdU) immunohistochemistry with Golgi impregnation. This straightforward and non-invasive approach enables the three-dimensional visualization and quantitative analysis of dendritic arborization and spine morphology in newborn neurons within the adult rodent brain.