Tracking the metabolism of selenium compounds in Spinacia oleracea using multiple stable isotope tracers and HPLC-(ID)-ICP-MS

This study demonstrates the use of selenium isotope tracers to evaluate and compare the accumulation and metabolic transformation of different selenium forms in a single experiment. By combining post-column IDMS with HPLC-ICP-MS, all selenium species in spinach were quantified without the need for commercial standards. This innovative methodology offers a comprehensive approach for exploring selenium metabolic pathways and holds potential for advancing research in plant fertilization, particularly when paired with advanced mass spectrometry techniques.