Identification and genome insights into Pyrenochaeta nobilis, a novel endophytic fungus isolated from Astragalus membranaceus with gray mold-control activity

Our findings provide the first evidence that <i>P. nobilis</i> could serve as a promising natural antagonist against <i>B. cinerea</i>, particularly in integrated disease management systems for tomato production in greenhouses. The high-quality genome sequence and the identification of key gene families lay a solid foundation for future research on the molecular mechanisms underlying the inhibitory activity of Pyrenochaeta spp. against <i>B. cinerea</i>. Further studies are needed to explore the practical application of <i>P. nobilis</i> in agricultural settings and to elucidate its mode of action at the molecular level.