Cardiac regeneration revisited: Enhanced cardiomyocyte differentiation and repair through composite microenvironments and small molecules

Our study presents a robust, scalable, and mechanistically informed platform that synergistically integrates ECM engineering with targeted signaling pathway modulation. By combining fibronectin-Matrigel composites with small-molecule-directed differentiation, this strategy provides a powerful tool for cardiac tissue engineering, disease modeling, high-throughput drug screening, and regenerative therapies, representing a significant step toward clinically relevant cardiac repair.