CRISPR with a Double Mismatch Guide RNA Enhances Detection Sensitivity for Low-Frequency Single-Base EGFR Mutation in Circulating Cell-Free DNA of Lung Cancer Patients
Our findings highlight the potential of the CRISPR/Cas12a-based mutation enrichment system as a powerful tool for detecting rare oncogenic mutations in liquid biopsy samples. This technique enhances diagnostic sensitivity and could be broadly applicable for the non-invasive detection of various genetic alterations in cancer and other diseases.
Authors
- Seunghun Kang (ORCID: https://orcid.org/0000-0001-6317-4321)
- Junho K. Hur (ORCID: https://orcid.org/0000-0003-3794-1149)
- Kyung Wook Been (ORCID: https://orcid.org/0000-0002-6946-2188)
- Woochang Hwang (ORCID: https://orcid.org/0000-0003-0489-3304)
- Sumin Hong (ORCID: https://orcid.org/0009-0004-8887-7874)
- Boksoon Chang (ORCID: https://orcid.org/0000-0003-1623-2685)
- Taegeun Bae
- G.K. Kim
Institutions
- Samsung Medical Center (KR)
- Hanyang University (KR)
Publication Details
- Journal
- Cancers
- Published
- 2025-10-16
- DOI
- https://doi.org/10.3390/cancers17203343
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- Kyung Hee University
- Korea Basic Science Institute
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea