Synthesis and Characterization of Silver Oxide Nanoparticles Using Lemon (Citrus limon) Leaves Extract: An Eco-Friendly Approach towards the Development of Potent Bioactive Antioxidant Agent

The primary objective of this study was to employ an eco-friendly approach for the synthesis of silver oxide nanoparticles (AgO NPs) using aqueous extracts derived from lemon (Citrus limon) leaves. This method offers numerous advantages, including biocompatibility, environmental sustainability, and resilience, making it an attractive alternative to conventional synthesis techniques. The synthesized AgO NPs underwent comprehensive analysis using Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Dynamic light scattering and Scanning Electron Microscopy-Energy Dispersive X-ray Analysis. Furthermore, the antioxidant potential of the synthesized AgO NPs was evaluated using 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid assays. These assays provide insights into the ability of the nanoparticles to scavenge free radicals, highlighting their potential applications in biomedical and environmental settings. Overall, the comprehensive characterization and evaluation of the synthesized AgO NPs underscore their potential as versatile nanomaterials with promising antioxidant potential, paving the way for further exploration in various fields of research and application.