Isolation and structure elucidation of Buchholzcine A, a novel steroidal cyanoformamide from the stem bark of <i>Buchholzia coriacae</i> Engler (<i>Capparacea</i>e)

Isolation and characterization was carried out on the stem bark of Buchholzia coriacea, commonly called wonderful kola. The plant have been used by traditional people to treat hypertension, headache, and various infections. The dried and powdered plant material was extracted with ethanol to get the crude extract which was partitioned between chloroform and n-hexane (1:1). The chloroform fraction was subjected to column chromatography for purification. Thin layer chromatography (TLC) was used to confirm the purity of the isolated compound. The isolated compound was observed as a golden yellow oil, gave a single spot at Rf 0.62 in n-hexane/chloroform/methanol (25:15:60). The isolated compound was characterized through joint interpretation of its FT-IR, 1HNMR, 13CNMR spectra, and 2-dimensional techniques including 1H-1H COSY, HMQC, and HMBC. Buchholzcine A, a steroidal Cyanoformamide was proposed, with IUPAC name: cyano-N- (12E, 14E, 18Z)-19-(hexadecahydro-10,13-dimethyl-1H-cyclopenta[α]phenanthren-17-yl) nonadeca-12,14,16,18-tetraenylformamide.