Effect of Chitosan and bioactive glass nanomaterials as intracanal medicaments on TGF-β1 release from intraradicular dentin

This study aimed to assess the influence of Bioactive glass nanoparticle (BAG-np) and Chitosan nanoparticle (CSNP) as intracanal medications compared with triple antibiotic paste (TAP) on the release of TGF-β1 from radicular dentin via enzyme-linked immunosorbent assay (ELISA). Forty dentin discs (1-mm thick) were horizontally cut from roots of 40 teeth.The samples were submerged in 20 ml of 1.5% NaOCL, followed by submersion in 20 ml 17% of EDTA, after which they were distributed equally into 2 experimental groups (I, II) and 2 control groups (III, IV) (10 samples each). Group I: (BAG-np), group II: (CSNP), group III: (TAP) (positive control) and group IV: nonmedicated (negative control). After 3 weeks, the medications were removed via Phosphate buffer saline (PBS), and the samples were subsequently submerged in PBS for 24 h at 37 °C. The medium from the samples was collected and used to measure the TGF-β1 concentration via ELISA. There was a statistically significant difference between the BAG-np, CSNP, and nonmedicated groups compared to the TAP group (P-value < 0.05). The highest value of released (TGF-β1) was recorded in the CSNP group, followed by the BAG-np group, then the nonmedicated group, whereas the lowest value was recorded in the TAP group. BAG-np and CSNP induce the release of TGF-β1after irrigation with 1.5% NaOCl and 17%EDTA, significantly greater than TAP. Accordingly, BAG-np and CSNP may be used as alternatives to TAP in regenerative endodontic procedures.