An optimized DNA extraction protocol for reliable PCR-based detection and characterization of grapevine flavescence dorée phytoplasma
HSV is a fast, reliable, and chemically low-risk DNA extraction method for FDp detection and characterization in grapevine. Its efficiency and simplicity make HSV ideal for large-scale diagnostics and early disease management.
Authors
- Domenico Rizzo (ORCID: https://orcid.org/0000-0003-2834-4684)
- Elisa Pellegrini (ORCID: https://orcid.org/0000-0001-9237-5341)
- Cristina Nali (ORCID: https://orcid.org/0000-0002-8228-0258)
- Lorenzo Cotrozzi (ORCID: https://orcid.org/0000-0002-4401-3896)
- Marco Carli (ORCID: https://orcid.org/0000-0003-1447-4947)
- Athos Pedrelli
- Alessandra Panattoni
Institutions
- University of Pisa (IT)
- University of Salento (IT)
- State Phytosanitary Administration (CZ)
- University of Florence (IT)
Publication Details
- Journal
- Plant Methods
- Published
- 2025-10-16
- DOI
- https://doi.org/10.1186/s13007-025-01460-y
- Citations
- 1
- Primary Topic
- Phytoplasmas and Hemiptera pathogens
- Type
- article
- Field-Weighted Citation Impact
- 1.75