In situ proximity ligation assay for analysing spatial interactions between ciliary proteins
The in situ PLA technique offers a powerful means of detecting protein proximities in subciliary compartments with high spatial resolution. This method supports the identification of novel protein interactions and contributes to a deeper understanding of subciliary architecture and its disruption in ciliopathies.
Authors
- Thorsten Pfirrmann (ORCID: https://orcid.org/0000-0002-9474-9535)
- Ulrich Rüther (ORCID: https://orcid.org/0000-0001-8184-7446)
- Christoph Gerhardt (ORCID: https://orcid.org/0000-0002-6340-549X)
Institutions
- University of Potsdam (DE)
- Heinrich Heine University Düsseldorf (DE)
Publication Details
- Journal
- BMC Molecular and Cell Biology
- Published
- 2025-10-16
- DOI
- https://doi.org/10.1186/s12860-025-00555-7
- Citations
- 1
- Primary Topic
- Genetic and Kidney Cyst Diseases
- Type
- article
- Field-Weighted Citation Impact
- 1.82