Analysis of the Correlation Between Depression-like Behaviors and Lipid Peroxidation in the Prefrontal Cortex of Mice: The Impact of Early Life Stress

<b>Objectives</b>: This study attempted to investigate whether early life stress (ELS) induces lipid peroxidation in the prefrontal cortex (PFC) of mice and its correlation with depression-like behavioral changes. <b>Methods</b>: A mouse ELS model was established via maternal separation. Depressive and anxiety-like behaviors were assessed using the forced swim test, sucrose preference test, tail suspension test, and open field test. The expression levels of GPX4, SLC3A2, SLC7A11, TFR1, and lipid peroxidation markers in the PFC of mice were measured and correlated with depression-like behavioral changes. <b>Results</b>: ELS induced depressive and anxiety-like behaviors in mice. The mRNA and protein expressions of GPX4, SLC3A2, and SLC7A11 were downregulated in the PFC of ELS mice; the mRNA level of TFR1 was upregulated but its protein level remained unchanged. In the PFC of ELS mice, the product of lipid peroxidation, malondialdehyde, was significantly increased, while the antioxidants, glutathione and superoxide dismutase, were significantly decreased. These markers were significantly correlated with sucrose preference or immobility time of the ELS mice. <b>Conclusions</b>: The study evidences that early life stress can induce lipid peroxidation in the PFC of mice and that the latter is closely associated with depression-like behaviors, hinting that ELS may induce depression via lipid peroxidation in the PFC. These findings may suggest a potential strategy for the treatment of early-stage stress-related depression.