LpxR and PagL expression in live attenuated auxotrophic Pseudomonas aeruginosa vaccines modulates lipid A reactogenicity in vitro while preserving immunogenicity
Lipid A modifications mediated by LpxR and PagL attenuated TLR4 signaling <i>in vitro</i> but were insufficient to reduce <i>in vivo</i> reactogenicity. Additional modifications or targeting of other toxic components may be required. This strategy may serve as an initial basis for optimizing live attenuated <i>P. aeruginosa</i> vaccines, although additional approaches will likely be necessary to achieve substantial improvements.
Authors
- Jesús Arenas (ORCID: https://orcid.org/0000-0002-8134-0693)
- Juán A. Vallejo (ORCID: https://orcid.org/0000-0002-7581-8654)
- Víctor Fuentes-Valverde (ORCID: https://orcid.org/0000-0002-1827-7003)
- Marina Oviaño (ORCID: https://orcid.org/0000-0001-5977-0024)
- Jesús Pérez‐Ortega (ORCID: https://orcid.org/0000-0002-8022-499X)
- Patricia Garcı́a (ORCID: https://orcid.org/0000-0002-0418-296X)
- Ana Candela (ORCID: https://orcid.org/0000-0003-0009-7116)
- Germán Bou (ORCID: https://orcid.org/0000-0001-8837-0062)
- Miriam Moscoso (ORCID: https://orcid.org/0000-0001-8977-3269)
- Rebeca Santamarina-Fernández
Institutions
- Universidade da Coruña (ES)
- Utrecht University (NL)
- Universidad de Zaragoza (ES)
- Instituto de Salud Carlos III (ES)
- Centro de Investigación Biomédica en Red de Salud Mental (ES)
- Centro de Investigación Biomédica en Red (ES)
- Centro de Investigación y Tecnología Agroalimentaria de Aragón (ES)
- Instituto de Investigación Biomédica de A Coruña (ES)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-10-16
- DOI
- https://doi.org/10.3389/fcimb.2025.1664169
- Citations
- 1
- Primary Topic
- Escherichia coli research studies
- Type
- article
- Field-Weighted Citation Impact
- 1.80