Efficacy of the antioxidant effect of l-lysine – (s)-2,6-diaminohexanoic acid 3-methyl-1,2,4-triazolyl-5-thioacetate in rabbits with contusional eye injury

Annotation. Ocular contusion creates conditions for the uncontrolled generation of free radicals, which in turn leads to a cascade of reactions associated with nitrosative stress. This process intensifies the initial tissue damage. Under these stressful conditions, the balance between the formation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) and the functioning of the body's antioxidant systems is disrupted. The purpose of this study was to use an enzyme-linked immunosorbent assay (ELISA) to investigate the effect of Angiolin and the reference drug (OMC-1 eye drops) on the levels of reduced and oxidized glutathione and NO levels, as a potential mechanism of their antioxidant action. The experiments were conducted on 20 male and female California rabbits weighing 3.0–3.5 kg. Experimental ocular contusion was induced in the rabbits by a pressurized stream of carbon dioxide using a gas-powered pneumatic pistol. The affected eye was immediately treated with 0.2 ml of the investigational eye drops, Angiolin-1%, three times a day for 10 days. The reference drug, OMC-1 eye drops (citicoline-2%), was also administered in the same manner. On the 10th day, a blood sample (0.5 ml) was taken from the superior ophthalmic vein for ELISA. Reduced and oxidized glutathione levels were determined fluorometrically. The data are presented as the mean value. A one-way analysis of variance (ANOVA) was used for normally distributed data, while the Kruskal-Wallis test was applied for non-normal distributions. For all analyses, differences were considered statistically significant at p < 0.05 (95%). The study revealed that Angiolin eye drops therapy contributed to the normalization of the glutathione link of the thiol-disulfide system. It increased the level of reduced glutathione by 68.6% (p<0.05) and decreased the concentration of oxidized glutathione by 50.7% (p<0.05). A decrease in NO overproduction was also observed (the level of stable NO metabolites decreased by 40.1% (p < 0.05)) compared to the control group. In terms of the magnitude of its effect on these parameters, Angiolin was demonstrably superior to OMC-1 drops.