Digital holographic microscopy for rapid bacteria segmentation and counting in microfluidic cartridges: basic considerations and limitations for diagnostic application
We successfully built a DHM system for counting suspended particles over a wide concentration range and for various microfluidic chamber heights. We also derived an initial framework for predicting and optimizing the performance of a given DHM system.
Authors
- Daniel Claus (ORCID: https://orcid.org/0000-0002-0602-9715)
- Florian Hausladen
- Rainer Wittig (ORCID: https://orcid.org/0000-0002-3735-9370)
- Igor Alekseenko (ORCID: https://orcid.org/0000-0001-8463-3304)
- Moaaz Rauf Nizami (ORCID: https://orcid.org/0000-0002-3890-3382)
- Damien P. Kelly (ORCID: https://orcid.org/0000-0001-7423-4801)
- Hussein Kamel
- Julian Schmid
Institutions
- Institute for Laser Technology in Medicine and Measurement Technique (DE)
Publication Details
- Journal
- Journal of Biomedical Optics
- Published
- 2025-10-15
- DOI
- https://doi.org/10.1117/1.jbo.30.10.106501
- Primary Topic
- Digital Holography and Microscopy
- Type
- article
- Field-Weighted Citation Impact
- 0.00