Optimization of Mitragyna speciosa (Korth.) Havil. extraction techniques

Context: This study was conducted to investigate the relationship between the use of appropriate temperature and time with the antioxidant activity of Mitragyna speciosa (Kratom) extract. Methods: Aqueous extract prepared at various time and temperature were tested using DPPH, TPC, FRAP, and MDA tests. Results: The most active extracts were obtained 60°C for more than one hour extraction. Conclusion: The extraction temperature and time for the preparation of M. speciosa extract are important parameters to ensure its high antioxidant potential activity and best extraction of phenolics.