Effects of Riparian Zone Width and Soil Depth: Soil Environmental Factors Drive Changes in Soil Enzyme Activity

Functioning as a critical ecotone between terrestrial and aquatic ecosystems, riparian zones exhibit soil enzyme activities that serve as key biomarkers of their nutrient cycling processes. However, despite considerable focus on riparian soil properties, the dynamics and underlying drivers of these enzymatic activities are not yet fully characterized. To this end, soils were systematically sampled across varying widths and depths from three representative riparian zones to quantify the driving forces of physicochemical properties on enzyme activity dynamics. The results showed that the soil enzyme activity was highest in the forest riparian zone and lowest in the farmland riparian zone, with average enzyme activities of 37.95 (μmol·g−1·h−1) and 26.85 (μmol·g−1·h−1), respectively. The width of the riparian zone changes the spatial distribution of soil enzyme activity. The soil enzyme activity is higher in the land edge area far from the river (profile-1) and lower in the water edge area near the river (profile-4), with average enzyme activities of 47.4384 (μmol·g−1·h−1) and 17.0017 (μmol·g−1·h−1), respectively. Moreover, soil water content (SWC) has a strong impact on enzyme activity changes. The increase in soil depth reduces soil enzyme activity, with enzyme activity in the 0–20 cm soil layer being 1.5 times higher than in the 20–50 cm soil layer. Meanwhile, the primary factors influencing changes in soil enzyme activity have gradually shifted from total nitrogen (TN), nitrate nitrogen (NO3-N), and soil organic carbon (SOC) to the sole control of SOC. Research has shown that human influence strongly interferes with soil enzyme activity in riparian zones. The width of the riparian zone and soil depth serve as key drivers of the spatial distribution of soil enzyme activity by modulating soil environmental factors. The patterns revealed in this study indicate that maintaining appropriate riparian zone width and reducing anthropogenic disturbances can enhance nutrient cycling dynamics at the micro-scale by increasing soil enzyme activity. This process is crucial for strengthening the riparian zone’s macro-level ecosystem services, particularly by effectively enhancing its capacity to sequester and transform nutrients like nitrogen and phosphorus from agricultural nonpoint sources, thereby safeguarding downstream water quality. Consequently, soil enzyme activity serves as a key indicator, providing essential scientific basis for assessing riparian health and guiding ecological restoration efforts.