Selenium‐Enriched Matcha Ameliorates Alcohol‐Induced Liver Injury by Modulating Cytochrome P450 Expression in Mice

ABSTRACT Selenium is an essential trace element for human health, and the selenium content in selenium‐enriched matcha meets the official criteria for selenium‐enriched foods in China. In the present work, hepatoprotective effects of selenium‐enriched matcha against alcohol‐induced liver injury were systematically investigated, and its mechanism was explored by label‐free proteomic technique for the first time. When fed with selenium‐enriched matcha, selenium was distributed in various organs and tissues (heart, liver, spleen, lung, kidney, serum, and muscle), and the selenium content in the liver was significantly higher than that in other organs and tissues ( p < 0.05). Levels of alanine transaminase, aspartate transaminase, total cholesterol, triglyceride, total bilirubin, and malondialdehyde in selenium‐enriched matcha intervention groups were lower than those in the alcohol model (AM) group, while levels of glutathione peroxidase, superoxide dismutase, and glutathione in selenium‐enriched matcha intervention groups were higher than those in the AM group. A total of 376 differentially expressed proteins (DEPs) were identified, in which 35 key DEPs were involved in 26 biological processes, 14 cellular components, and 14 molecular functions. Intervention with selenium‐enriched matcha notably downregulated cytochrome P450 (Cyp2c40 and Cyp2c54), which specifically modulated pathways of chemical carcinogenesis‐DNA adducts, arachidonic acid, and retinol metabolisms. These results provide evidence for the use of selenium‐enriched matcha in alleviating alcohol‐induced liver injury.