Evaluation of Wood Decay and Identification of Fungi Found in the USS Cairo, a Historic American Civil War Ironclad Gunboat

Studies of microbial degradation of historic woods are essential to help protect and preserve these important cultural properties. The USS <i>Cairo</i> is a historic Civil War gunboat and one of the first steam-powered and ironclad ships used in the American Civil War. Built in 1861, the ship sank in the Yazoo River of Mississippi in 1862 after a mine detonated and tore a hole in the port bow. The ship remained on the river bottom and was gradually buried with sediments for over 98 years. After recovery of the ship, it remained exposed to the environment before the first roofed structure was completed in 1980, and it has been displayed under a tensile fabric canopy with open sides at the Vicksburg National Military Park in Vicksburg, Mississippi. Concerns over the long-term preservation of the ship initiated this investigation to document the current condition of the wooden timbers, identify the fungi that may be present, and determine the elemental composition resulting from past wood-preservative treatments. Micromorphological characteristics observed using scanning electron microscopy showed that many of the timbers were in advanced stages of degradation. Eroded secondary cell walls leaving a weak framework of middle lamella were commonly observed. Soft rot attack was prevalent, and evidence of white and brown rot degradation was found in some wood. DNA extraction and sequencing of the ITS region led to the identification of a large group of diverse fungi that were isolated from ship timbers. Soft rot fungi, including <i>Alternaria</i>, <i>Chaetomium</i>, <i>Cladosporium</i>, <i>Curvularia</i>, <i>Xylaria</i> and others, and white rot fungi, including <i>Bjerkandera</i>, <i>Odontoefibula</i>, <i>Phanerodontia</i>, <i>Phlebiopsis</i>, <i>Trametes</i> and others, were found. No brown rot fungi were isolated. Elemental analyses using induced coupled plasma spectroscopy revealed elevated levels of all elements as compared to sound modern types of wood. High concentrations of boron, copper, iron, lead, zinc and other elements were found, and viable fungi were isolated from this wood. Biodegradation issues are discussed to help long-term conservation efforts to preserve the historic ship for future generations.