Synergistic effect of DR5-targeted capsules loaded with doxorubicin in drug-resistant 3D tumour spheroids
Mean capsule size was 320 ± 90 nm (PDI 0.39), mean ζ-potential was +29 ± 5 mV. The encapsulation efficiencies (ЕЕ) of DOX and DR5-B were 85 ± 7% and 80 ± 4%, with loading capacities (LC) of 9 ± 2 w/w% and 145 ± 40 w/w%, respectively. Shrunken capsules exhibited 3.5-fold higher internalisation than non-shrunken ones. DR5-B increased capsule accumulation by 1.8-fold. The capsules showed synergistic cytotoxicity in DR5-B-resistant MCF-7 spheroids (IC50 227 ± 13 ng/mL), being non-toxic to fibroblasts.
Authors
- А. М. Gileva (ORCID: https://orcid.org/0000-0001-8220-0580)
- Т. В. Букреева (ORCID: https://orcid.org/0000-0001-7800-1490)
- В. В. Артемов (ORCID: https://orcid.org/0000-0002-5253-8481)
- Anne V. Yagolovich (ORCID: https://orcid.org/0000-0003-3145-3726)
- Leyli Kurbanova (ORCID: https://orcid.org/0000-0002-9941-3694)
- Marine E. Gasparian (ORCID: https://orcid.org/0000-0003-2308-2574)
- S. V. Burov (ORCID: https://orcid.org/0000-0003-3607-5680)
- Daria B. Trushina (ORCID: https://orcid.org/0000-0001-9871-8050)
- Тatiana Pallaeva (ORCID: https://orcid.org/0000-0003-4076-5078)
- Елена Марквичева (ORCID: https://orcid.org/0000-0001-6652-3267)
- В. А. Олейников (ORCID: https://orcid.org/0000-0003-4623-4913)
Institutions
- D. Mendeleyev University of Chemical Technology of Russia (RU)
- Lomonosov Moscow State University (RU)
- Kurchatov Institute (RU)
- Institute of Cytology (RU)
- Institute of Bioorganic Chemistry (RU)
- A.V. Shubnikov Institute of Crystallography (RU)
- Institute of Bioorganic Chemistry (BY)
- Moscow State University (TJ)
- Depomed (United States) (US)
- Thermo Fisher Scientific (Norway) (NO)
Publication Details
- Journal
- Journal of Microencapsulation
- Published
- 2025-10-03
- DOI
- https://doi.org/10.1080/02652048.2025.2570651
- Citations
- 1
- Primary Topic
- Nanoparticle-Based Drug Delivery
- Type
- article
- Field-Weighted Citation Impact
- 0.60