Evaluation of the biologically active substances influence on the physiological processes optimization in potential sturgeon broodstock

The article presents the results of a study of the influence of the mineral and vitamin supplement E-selenium on the functioning of the reproductive system of the Russian-Lena sturgeon hybrid that has not reached sexual maturity, and the broodstock of the sterlet × beluga hybrid. To do this, we studied a complex of physiological and biochemical blood parameters (hemoglobin, ESR, total protein, β-lipoproteins, cholesterol), reflecting the intensity and direction of metabolism, as well as the state of the gonads based on morphological characteristics using ultrasound scanning and analysis of histological preparations of the gonads. Two series of experiments were carried out with the addition of the E-selenium drug to the feed: experiments with immature fish – with low (300 μg/kg) and high (2 000 μg/kg of feed) concentrations and experiments with mature hybrids, to the feed of which the drug was added at the rate of 1 mg of selenium and 100 mg of vitamin E / kg of feed. The use of the E-selenium drug in the first series of experiments did not contribute to the accelerated development of the generative function, which was confirmed by the level and direction of metabolism, but it had a positive effect on the level of mass accumulation in fish in the experimental version at high concentrations of the E-selenium drug. The use of the drug in the feed of sexually mature sterlet × beluga hybrids revealed its positive effect on the reproductive system. An increase in the intensity of generative metabolism, the marker of which was the content of total protein and β-lipoproteins in the blood, contributed to an increase, in comparison with the control, in the proportion of mature females (IV SZG) by two times and a decrease in the proportion of females remaining at stage II of maturation by 1.8 times, in relation to the control. In the case of the control, signs of a slight stress state of the fish were found, which did not appear in the experimental batch of fish.