Cordypyridones E–J: Antibiofilm 2-Pyridone Alkaloids from the Nematode Antagonistic Fungus <i>Laburnicola nematophila</i>
In the course of biochemical prospection of <i>Laburnicola nematophila</i> isolated from eggs of the plant-parasitic cyst nematode <i>Heterodera filipjevi</i>, eight 2-pyridone alkaloids were isolated from its solid-state BRFT cultures and identified as six previously undescribed, cordypyridones E-J (<b>1</b>-<b>6</b>), and two known congeners, cordypyridones C (<b>7</b>) and D (<b>8</b>). The structures and absolute configurations of all isolated compounds were elucidated through HR-ESI-MS, 1D/2D NMR spectroscopy, and TDDFT-ECD calculations. Compound <b>4</b>, bearing a rare <i>N</i>-hydroxy-2-pyridone moiety structurally related to that of PF1140, exhibited broad-spectrum bioactivity in cytotoxicity and antimicrobial assays. Compounds <b>5</b> and <b>6</b> demonstrated potent antibiofilm activities against <i>Staphylococcus aureus</i>, reducing its biofilm formation by almost 50% at 0.25 and 7.8 μg/mL, respectively.
Authors
- Hedda Schrey (ORCID: https://orcid.org/0000-0003-0079-8908)
- Jan‐Peer Wennrich (ORCID: https://orcid.org/0000-0002-4309-7290)
- S. Fushimi
- Marc Stadler (ORCID: https://orcid.org/0000-0002-7284-8671)
- Mahmoud A. A. Ibrahim (ORCID: https://orcid.org/0000-0003-4819-2040)
- Wolfgang Maier (ORCID: https://orcid.org/0000-0002-2531-6998)
- Sherif S. Ebada (ORCID: https://orcid.org/0000-0002-2753-0031)
- Samad Ashrafi (ORCID: https://orcid.org/0000-0003-0426-7039)
- Caren Holzenkamp (ORCID: https://orcid.org/0009-0008-8439-3230)
Institutions
- Ain Shams University (EG)
- Julius Kühn-Institut (DE)
- University of Applied Science and Technology (IR)
- University of the Free State (ZA)
- Czech Academy of Sciences, Institute of Animal Physiology and Genetics (CZ)
- College of Applied Sciences, Nizwa (OM)
- Czech Academy of Sciences, Institute of Microbiology (CZ)
- Helmholtz Centre for Infection Research (DE)
- Institute of Molecular Genetics (RU)
- Computational Physics (United States) (US)
- Pediatrics and Genetics (US)
- Pharmac (NZ)
- Toyama Prefectural University (JP)
- Minia University (EG)
- Technische Universität Braunschweig (DE)
- University of KwaZulu-Natal (ZA)
Publication Details
- Journal
- Journal of Natural Products
- Published
- 2025-10-14
- DOI
- https://doi.org/10.1021/acs.jnatprod.5c00768
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00