Quantifying human pluripotent stem cell attributes with population balance modeling

The work provides a first account of the derivation of rate distributions - rather than population averages - of stem cell physiological properties including division and change in OCT4 content. This enables the implementation of modeling frameworks for the rigorous quantitative description of hPSC populations that is important for addressing fundamental biological questions about pluripotency and differentiation, and critical in the biomanufacturing of hPSC-based therapeutics.