Control of Aedes albopictus populations by silencing of the vesicular GABA transporter (vgat) and the vesicular monoamine transporter (vmat) genes using recombinant Chlorella shRNA
Recombinant Chlorella expressing vgat and vmat shRNAs demonstrated strong lethality, high specificity, and good safety against both larval and adult Ae. albopictus. Oral delivery of shRNAs effectively knocked down mosquito target genes, providing a convenient approach for functional studies of mosquito genetics. Notably, this study is an important addition to the use of recombinant microalgae as a mosquito biocide.
Authors
- J. Gao (ORCID: https://orcid.org/0000-0003-4619-9548)
- Xiaodan Huang (ORCID: https://orcid.org/0000-0001-8741-2785)
- Xiaowen Fei (ORCID: https://orcid.org/0000-0002-4228-6516)
- Juncai Li (ORCID: https://orcid.org/0000-0002-3169-8646)
- Xiaodong Deng (ORCID: https://orcid.org/0000-0002-6938-1214)
- Dafeng Xu (ORCID: https://orcid.org/0000-0002-8839-8433)
- Chunmei Xue
Institutions
- Chinese Academy of Tropical Agricultural Sciences (CN)
- Jiamusi University (CN)
- Tropical Crops Genetic Resources Institute (CN)
- Zhanjiang Experimental Station (CN)
- Hainan Medical University (CN)
Publication Details
- Journal
- Parasites & Vectors
- Published
- 2025-10-14
- DOI
- https://doi.org/10.1186/s13071-025-07053-x
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hainan Province
- Hainan Medical University