The Na <sup>+</sup> /K <sup>+</sup> ‐ATPase β1 Subunit is a Kidney ADP‐Ribosyl Cyclase
ADP-ribosyl cyclase (ARC) produces a Ca<sup>2+</sup>-mobilizing second messenger, cyclic ADP-ribose (cADPR), from NAD. In this study, we purified an ARC from rat kidney, which was identified as the β1 subunit of Na, K-ATPase (Atp1b1). Recombinant Atp1b1 exhibited ARC activity and generated cADPR. Knockdown of Atp1b1 in mouse mesangial cells (MES-13) inhibited angiotensin II (AngII)-induced cADPR synthesis and the subsequent long-lasting Ca<sup>2+</sup> signals. These findings demonstrate that Atp1b1 is coupled with the AngII receptor and is activated to produce cADPR, thereby mediating Ca<sup>2+</sup> signaling in the kidney.
Authors
- Tae‐Sik Nam
- Uh‐Hyun Kim (ORCID: https://orcid.org/0000-0003-3304-7190)
- Seong‐Kyu Choe (ORCID: https://orcid.org/0000-0002-2102-973X)
Institutions
- Wonkwang University (KR)
Publication Details
- Journal
- The FASEB Journal
- Published
- 2025-10-14
- DOI
- https://doi.org/10.1096/fj.202502065rr
- Primary Topic
- Calcium signaling and nucleotide metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- National Research Foundation of Korea