Enabling mechanistic studies of EVs in vivo: a protocol for isolation and cell-specific labelling in larval zebrafish
These advances provide a versatile toolkit for mechanistic studies of EV function in vivo. The broad availability of cell-type-specific Gal4 driver lines in zebrafish and Drosophila will now allow researchers to trace EV dynamics from virtually any cell type, while our isolation protocol enables rigorous, quantitative EV analyses across developmental and pathological contexts.
Authors
- Ezgi Kıyga (ORCID: https://orcid.org/0000-0002-1325-8268)
- Dirk Sieger (ORCID: https://orcid.org/0000-0001-6881-5183)
- Guillaume van Niel (ORCID: https://orcid.org/0000-0002-8651-9705)
- Julie Mazzolini (ORCID: https://orcid.org/0000-0001-9347-5635)
- Kayleigh M. Reid
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers (FR)
- UK Dementia Research Institute (GB)
- Nantes Université (FR)
- University of Edinburgh (GB)
Publication Details
- Journal
- Cell Communication and Signaling
- Published
- 2025-10-14
- DOI
- https://doi.org/10.1186/s12964-025-02433-3
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Brain Research UK