Antimicrobial Activity of Papay (Vasconcellea Pubescens), Custard Apple (Annona Squamosa) and Tulsi (Ocimum Sanctum Linn.) Leaf Extract Against E. Coli and Pseudomonas

Papaya, Custard apple and Tulsi plant have many restorative properties. The leaf extract of this plant seems to have incredible antibacterial, antiviral, and anti- inflammatory activity because it contains dynamic components such as Alkaloids, Glycosides, Tannins, Saponins, Flavonoids, Steroid compounds. Both E. Coli and Pseudomonas are gram positive and Gram-negative bacteria. They can cause Serious food poisoning and effect on human health and animals. Each Plant extract was prepared with the help of Soxhlet apparatus. Pouring method used for inoculation and well diffusion agar method was used to determine the antibacterial activity of the plant extract. New specific pathogens were plated on Mueller Hinton agar plates. The proximity of a clear restriction zone around Pseudomonas and E. coli indicates that they are valuable as antimicrobial agents. Finding of the result on E.coli all three leaves extract papaya leaves (1.4 cm.) tulsi leaves extract (2.5 cm.) and custard leaves (1.8 cm.) dimeter of zones was visible and in the case of pseudomonas papaya leaves extract shows negative effects. Tulsi and custard apple shows antimicrobial activity on pseudomonas and clear zone is visible.