Uncoupling protein production from growth: different strategies for intracellular and secreted proteins in yeast
Our results demonstrate that promoter selection plays a critical role in production performance under slow growing conditions. Moreover, it highlights that optimising intracellular and extracellular recombinant protein production requires distinct, strategy-specific approaches.
Authors
- Markus M.M. Bisschops (ORCID: https://orcid.org/0000-0003-2963-3920)
- Nuran Temelli (ORCID: https://orcid.org/0000-0002-8371-525X)
- Ruud A. Weusthuis (ORCID: https://orcid.org/0000-0001-6452-5701)
- Willem H. Baris
Institutions
- Wageningen University & Research (NL)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-10-14
- DOI
- https://doi.org/10.1186/s12934-025-02848-0
- Citations
- 1
- Primary Topic
- Fungal and yeast genetics research
- Type
- article
- Field-Weighted Citation Impact
- 0.81