Pomegranate extract cream modulates TGF-beta and IL-6 in wistar rats with excision wounds

Wound healing is a complex, multifactorial process, and disruptions at any stage can delay recovery. Pomegranate peel extract has been traditionally used to accelerate wound healing, with topical applications of natural products preferred due to their safety and availability compared to synthetic agents. This study evaluated the effects of pomegranate extract cream (Punica granatum) on TGF-beta and IL-6 levels in Wistar rats with excision wounds. An in vivo experimental design was applied to 48 rats divided into 12 groups, with analyses performed on day 3 and day 7. TGF-beta and IL-6 levels were measured using the enzyme-linked immunosorbent assay (ELISA). The results showed a significant reduction in mean IL-6 levels and a significant increase in mean TGF-beta levels on both day 3 and day 7 (One-Way ANOVA, p<0.05). The 20% pomegranate extract cream demonstrated the greatest effect, reducing IL-6 and enhancing TGF-? expression, with outcomes comparable to bioplacenton treatment on day 7. These findings suggest that pomegranate extract cream may promote wound healing by modulating inflammatory and reparative molecular mediators.