The Role of Serum Interleukins and PTPN22 in the Pathogenesis and Progression of Immune Thrombocytopenia

Background: Immune thrombocytopenia (ITP) is a common acquired hematologic autoimmune illness. Objectives: this work goals to examine the role of interleukins (ILs) and polymorphisms of PTPN22 gene in the progression and pathogenesis of ITP. Methods: 50 ITP patients were included. Human serum levels of antinuclear antibodies (ANA), anti-platelet antibody (PA-Ab) and IL-40 were measured using enzyme linked immunosorbent assay (ELISA). Gene expression of Protein tyrosine phosphatase non-receptor type 22 (PTPN22) were determined using Real-time polymerase chain reaction (RT-PCR), while the sequencing of this gene was determined using Sanger method and genotyping was detected using conventional PCR. Result: No significant variations were observed in serum level of ANA, PA-Ab and IL-40, PTPN22 gene expression and its single nucleotide polymorphism (rs2488457) in acute and chronic cases of ITP.