Evaluating the Impact of Mineral Nutrient Concentration and Substrate Volume on the Development of Three Annual Coastal Plant Species

Soil mineral nutrient heterogeneity is a distinctive characteristic of coastal habitats, yet its impact on plant growth and development remains uncertain. The objective of the present study was to establish an experimental system for evaluating the influence of mineral nutrient availability on the development of three distinct short-lived wild coastal plant species: Phleum arenarium, Plantago coronopus, and Ranunculus sceleratus. These plants were cultivated in containers of different volumes employing an inert substrate with varying proportions of commercial garden soil in controlled conditions. Low mineral nutrient concentration served as a factor inhibiting plant vegetative growth for both P. arenarium and R. sceleratus plants, albeit with a substrate volume-dependent effect. In contrast, P. coronopus exhibited relatively low root biomass and exhibited minimal susceptibility to alterations in mineral nutrient concentration. Conversely, proportional allocation to roots decreased with increasing mineral nutrient concentration, mirroring the pattern observed for P. arenarium. Notably, for R. sceleratus, this effect was pronounced only at a high substrate volume. Furthermore, allocation to roots decreased with increasing substrate volume, but this occurred only at a high mineral nutrient concentration. The substrate, similar to that in coastal habitats, incorporated quartz sand with varying proportions of mineral-rich organic matter, providing comparable plant-available mineral concentrations for analyzing the effects of nutrient concentration, substrate volume, and genetic variability on plant growth and development. For future experiments, a wider range of mineral concentrations and more individual concentrations should be used to assess mineral availability more realistically.